Biology Study Guide Answer Key
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Modern Biology Study Guide Answer
1) Isolate plasmid DNA from bacterial cells and DNA from hummingbird cells. The hummingbird DNA contains the gene of interest 2) Cut both DNA samples with the same restriction enzyme, one that makes a single cut within the lacZ gene and many cuts within hummingbird DNA 3)Mix the cut plasmids and DNA fragments. Some join by base pairing; add DNA ligase to seal them together. The products are recombinant plasmids and many non recombinant plasmids 4) Mix the DNA with bacterial cells hat have a mutation in their own lacZ gene. Some cells take up a recombinant plasmid or other DNA molecule by transformation 5) Plate the bacteria on agar containing ampicillin and X-gal, a molecule resembling lactose. Incubate until colonies grow.